EMBO Practical Course on ubiquitin and SUMO
EMBO Practical Course on ubiquitin and SUMO
September 12th-19th, 2008
About the course:
The objective of this course is to teach a wide range of state of the art methods for the in vitro and in vivo functional analysis of ubiquitylation and sumoylation.
Step by step protocols will be covered in lectures and practical experiments regarding questions such as: i) is the protein of interest modified in vivo/in vitro; ii) which E2 / E3 enzyme is involve; iii) how can one identify the modification site; iv) does modification alter stability, localisation or interactions.
Practical experiments include, e.g., in vitro and in vivo modifiation reactions, analysis of non-covalent interactions with ubiquitin and SUMO, enrichment of modified species from cells, and a short course on bioinformatic tools for the prediction of modification sites, recognition motifs and interacing partners. Some experiments will allow researchers to bring their own samples, e.g., purified protein for in vitro sumoylation.
Lectures will cover a wider range of methods that can be applied to study SUMO and ubiquitin in diverse cellular processes including, e.g., endocytosis, signal transduction, nuclear transport, and DNA repair.Designated times for informal discussions as well as shared meals will provide ample opportunity for interactions with the teachers and instructors.
Lecturers:
Maria Masucci - The ubiquitin-proteasome system in EBV infection and oncogenesis
Simona Polo - Ubiquitin in EGF-Receptor Signaling
Harald Stenmark - Ubiquitin in endocytosis
Claudio Joazeiro - Functional genomic analysis of ubiquitin ligases
Kay Hofmann - A bioinformatical approach to the ubiquitin system
Ivan Dikic - Ubiquitin and SUMO as interaction modules
Aaron Ciechanover - Special Lecture - The Ubiquitin Proteasome System
Helle Ulrich - Ubiquitin and SUMO in budding yeast
Frauke Melchior - SUMOylation in mammalian cells
Claudio Joazeiro - Ubiquitylation in mitochondrial dynamics and signaling
Experiments - Tutors:
Simona Polo - Ubiquitylation in vitro and in vitro (mammalian cells)
Frauke Melchior - In vitro SUMOylation (bring your own protein)
Helle Ulrich - Detection of ubiquitin and SUMO targets in yeast
Ivan Dikic - Non-covalent binding of Ub and Ubls to their binding domains
Kay Hofmann - A bioinformatical approach to the ubiquitin system
Organizers:
Ivan Dikic, Frauke Melchior, Simona Polo, Janos Terzic and Helle Ulrich
Cosponsor:
