EMBO Practical Course on Ubiquitin and SUMO
Split, 29 July – 4 August, 2010.
Organizer: Helle Ulrich, Ivan Dikic, Frauke Melchior, Simona Polo
The objective of this course is to present state-of-the-art methodology for the analysis of ubiquitination and sumoylation. This will involve practical experiments demonstrating the use of modifying enzymes and ubiquitin receptors in vitro, the identification and analysis of in vivo targets in mammalian cells and yeast, and a tutorial on predictive and search tools for the bioinformatical analysis of components of the ubiquitin system. Lectures by experts will convey a wide range of background knowledge, covering both the basics of the ubiquitin and SUMO systems and applications of proteomics, mass spectrometry and large genomic screens. However, the emphasis will be on methodology and practical approaches.